|
KMID : 0606920150230050442
|
|
Biomolecules & Therapeutics
2015 Volume.23 No. 5 p.442 ~ p.448
|
|
|
Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo
|
|
Park Jin-Sung
Lee Hyun-Jae
Lee Dong-Yeong
Jo Ho-Seung
Jeong Jin-Hoon
Kim Dong-Hee
Nam Dae-Cheol
Lee Choong-Jae
Hwang Sun-Chul
|
|
|
Abstract
|
|
|
|
We evaluated the chondroprotective effects of wogonin by investigating its effects on the gene expression and production of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as on production of MMP-3 in the rat knee. Rabbit articular chondrocytes were cultured in a monolayer, and RT-PCR was used to measure interleukin-1β (IL-1β)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and type II collagen. In rabbit articular chondrocytes, the effects of wogonin on IL-1β-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of wogonin on MMP-3 protein production was also examined in vivo. In rabbit articular chondrocytes, wogonin inhibited the expression of MMP-3, MMP-1, MMP-13, and ADAMTS-4, but increased expression of type II collagen. Furthermore, wogonin inhibited the production and proteolytic activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.
|
|
|
KEYWORD
|
|
|
Osteoarthritis, Wogonin, Chondrocyte, Metalloproteinase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|